THE IMPORTANCE OF EVs
Xu et al. (2021) Front. Cardiovasc. Med.
Combination biomarkers
EVs carry multiplexed molecular signatures with potential utility for early cancer detection via liquid biopsy.
EV ANALYSIS
See the EVs others miss.
Uncover combination biomarker signatures for earlier cancer detection.
THE CHALLENGE
Most EVs go unseen
Most EVs are <100 nm—below the detection limits of conventional macrofluidic nanoscale flow cytometers.
THE dFC SOLUTION
dFC detects even the smallest EVs
100% single-fluorophore detection efficiency enables detection far below conventional limits.
See the publicationSINGLE-PARTICLE ANALYSIS
Single-EV analysis by dFC:
- 12-channel biomarker analysisSimultaneous detection of multiple EV surface and cargo markers.
- EV phenotypingIdentify and characterize distinct EV subpopulations.
- Absolute concentrations of EV subpopulationsDirect, quantitative measurements.
- Absolute quantitation of biomarker copy numbersSingle-molecule sensitivity enables copy number measurements by digital deconvolution.
- EV sizing based on a membrane dyeDetermine EV size distributions using a lipid membrane dye.
SINGLE-PARTICLE ANALYSIS
Phenotyping individual EVs
With 4 excitation lasers and 12 color channels, dFC can unmask the biological heterogeneity of biomedical nanoparticles such as EVs in a single experiment—down to the smallest nanoparticle.
SINGLE-PARTICLE ANALYSIS
Absolute concentration measurements
Direct counting of fluorescent antibodies or EV subpopulations
By detecting every molecule that flows through the channel, dFC achieves absolute quantitation of the concentration of fluorescently-labeled biomolecules or EV subpopulations.
SINGLE-PARTICLE ANALYSIS
Absolute quantitation of biomarkers
The ultra-high sensitivity of dFC enables single-molecule counting of protein and nucleic acid biomarker copy numbers associated with each EV—without external calibration.
A CLOSER LOOK
Follow an EV through the dFC PanFlow™ Chip.
